4. Quick Tour of Cytoscape¶
This chapter describes the basic layout and mechanics of using Cytoscape. If you would prefer to learn by doing, consider starting with the Basic Expression Analysis Tutorial chapter.
4.1. Welcome Screen¶
When you start Cytoscape, you can access basic functions from the Welcome Screen:
The Welcome Screen is designed to access commonly used features of Cytoscape including:
- Create new network
- Import network
- From file
- From public database
- Import interactome for model organisms
- Open recently used session file
4.2. Basic Features¶
When a network is loaded, Cytoscape will look similar to the image below:
Most functionalities are self-explanatory, but we’ll go through a concise explanation for clarity. The main window here has several components:
- The Menu Bar at the top (see below for more information about each menu).
- The Tool Bar, which contains icons for commonly used functions. These functions are also available via the menus. Hover the mouse pointer over an icon and wait momentarily for a description to appear as a tooltip.
- The Network Panel (Network tab of the Control Panel, top left). This contains an optional network overview pane (shown at the bottom left).
- The main Network View Window, which displays the network.
- The Table Panel (bottom right panel), which displays columns of selected nodes and edges and enables you to modify the values of column data.
The Network Panel and Table Panel are dockable tabbed Panels. You can undock any of these panels by clicking on the Float Window control in the upper-right corner of the CytoPanel. This is useful when you want assign the network panel as much screen space as possible. To dock the window again, click the Dock Window icon . Clicking the Hide Panel icon will hide the panel; this can be shown again by choosing View → Show and selecting the relevant panel.
If you click this control, for example on the Table Panel, you will now have two Cytoscape windows, the main window, and a new window labeled Table Panel, similar to the one shown below. A popup will be displayed when you put the mouse pointer on a cell.
Note that Table Panel now has a Dock Window control. If you click this control, the window will dock onto the main window. For more information on the panels in Cytoscape, see the Panels section.
Cytoscape also has an edit functionality that enables you to build and modify networks interactively within the network canvas. To edit a network, just right-click on the open space of network window, select the menu item Add → Node, a new node will be added to the canvas. To add an edge, right click on a node, choose the menu item Edit → Add Edge. Then select the target node, a new edge will be added between the two nodes. In a similar way annotation objects can be added; pictures, shapes or textboxes; much like in MS PowerPoint or similar software. Detailed information on network editing can be found in the Editing Networks section.
4.4. Network Management¶
Cytoscape allows multiple networks to be loaded at a time, either with or without a view. A network stores all the nodes and edges that are loaded by the user and a view displays them.
An example where a number of networks have been loaded is shown below:
The network manager (in Control Panel) shows the networks that are loaded. Clicking on a network here will make that view active in the main window, if the view exists. Each network has a name and size (number of nodes and edges), which are shown in the network manager. If a network is loaded from a file, the network name is the name of the file.
Some networks are very large (thousands of nodes and edges) and can take a long time to display. For this reason, a network in Cytoscape may not contain a “view”. Networks that have a view are in normal black font and networks that don’t have a view are highlighted in red. You can create or destroy a view for a network by right-clicking the network name in the network manager or by choosing the appropriate option in the Edit menu. You can also destroy previously loaded networks this way.
Certain operations in Cytoscape will create new networks. If a new network is created from an old network, for example by selecting a set of nodes in one network and copying these nodes to a new network (via the File → New → Network option), it will be shown immediately follows the network that it was derived from.
Network views can also be detached (undocked) from the main Cytoscape window. When detached, a view window can be dragged to another monitor, resized, maximized and minimized by using the normal window controls for your operating system. Notice, however, that closing a view window does not destroy it, but simply reattaches it to the Cytoscape window.
Arrange Network Windows¶
When you have detached network view windows, you can arrange them by selecting one of these options under View → Arrange Network Windows:
Side by Side